久久精品精品,国产999久久,欧美 国产 日韩精品,请久久久久久

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-5974 SNU-16 人胃癌細(xì)胞 腹水

CRL-5974 SNU-16 人胃癌細(xì)胞 腹水

簡要描述:CRL-5974 SNU-16 人胃癌細(xì)胞 腹水,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-07-25
  • 訪  問  量:2131

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-5974 SNU-16 人胃癌細(xì)胞 腹水

ATCC® Number: CRL-5974™ Price: $425.00

Designations: SNU-16

Depositors: J Park

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-5974 SNU-16 人胃癌細(xì)胞 腹水

Isolation: Isolation date: 1987

Receptors: vasoactive intestinal peptide (VIP), expressed [23078]

Oncogene: myc +; erb B2 +

Antigen Expression: Blood Type A; Rh +

The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,12

D16S539: 11,13

D5S818: 10,13

D7S820: 12

THO1: 6,9

TPOX: 11

vWA: 16

Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.

Age: 33 years

Gender: female

Ethnicity: Asian

Comments: SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 27 hrs

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products




ATCC® Number: CRL-5974™ Price: $425.00

Designations: SNU-16

Depositors: J Park

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens (human)

Morphology: epithelial

CRL-5974 SNU-16 人胃癌細(xì)胞 腹水

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1987

Receptors: vasoactive intestinal peptide (VIP), expressed [23078]

Oncogene: myc +; erb B2 +

Antigen Expression: Blood Type A; Rh +

The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,12

D16S539: 11,13

D5S818: 10,13

D7S820: 12

THO1: 6,9

TPOX: 11

vWA: 16

Cytogenetic Analysis: This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.

Age: 33 years

Gender: female

Ethnicity: Asian

Comments: SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive. SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 27 hrs

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
九色精品人妻在线| 九九九九九九日产操逼视频| 大鸡巴插进小嫩逼视频| 碰碰电影天堂| 后入性感人妻| 日本三级 a| 好看不卡Av| 6050一级久久精品| 粉嫩AⅤ麻豆一区二区三区| 骚妻自拍| 欧美乂乂乂BBBB一区二区| 中文字幕人妻视频在线| 国产欧美日韩中文第一页| 强干内射人妻少妇嫩穴| AV看片福利| 欧美日韩在线观看123| 91精品92| 日韩欧美精品,一区,二区| 日本精品久久久中文字幕| 色色色迷迷色色色综合色色色| AV包浆在| 日韩字幕 欧美精品| 免费三区黄色电影网站| 献妻一区二区三区| 日韩性欧美| 99久久精品国产高清毛片| 大陆免费黄色录像| 亚洲国产无码在线| 男男同性恋精品久久久久| 西欧你懂的精品一区二区| 青春超碰在线porn| 亚洲一二三区视频在线观看| 一区二区88| 日韩人妻福利在线资源| 又粗又猛又爽的视频| 久久国产亚洲激情在线| 亚洲天堂无码| 夜夜姓夜夜爽| 精品华人在线看| 六月亭亭激情四射久久| 色呦呦中文字幕在线观看电影|