久久精品精品,国产999久久,欧美 国产 日韩精品,请久久久久久

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>倉(cāng)鼠肺細(xì)胞>CRL-1657R 1610 倉(cāng)鼠肺細(xì)胞

R 1610 倉(cāng)鼠肺細(xì)胞

簡(jiǎn)要描述:CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-1657
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-05-25
  • 訪  問(wèn)  量:2788

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件


CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞 的詳細(xì)介紹

CRL-1657

ATCC® Number:



Shipped:  frozen CRL-1657™

Designations:  R 1610

Depositors:   M Rosenberg

Biosafety Level: 1

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus (hamster, Chinese)

Morphology: fibroblast



Source: Organ: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host

Gender:  male

Comments: This line is a clonal derivative of V79 cells (see also V79-4, ATCC CCL-93) that had been treated with ethylmethanesulfonate.

The cells are deficient in galactokinase and are resistant to 2-deoxygalactose and 8-azaguanine.

This is a Chinese hamster somatic cell line.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.



Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liqid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-93

References: 10111: Schumperli D, et al. Efficient expression of Escherichia coli galactokinase gene in mammalian cells. Proc. Natl. Acad. Sci. USA 79: 257-261, 1982. PubMed: 6281760

25665: Thirion JP, et al. Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose. Genetics 83: 137-147, 1976. PubMed: 1269916

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
香蕉久久久久久久特片| 色悠悠久久久免费视频| 精品一区二区十三区| 一区二区三区乱伦鲁| 2区日韩免费| 亚洲一区搜索久久| 污污 网站在线一区| 国产成人久久77777精品,| 香蕉視頻成人黃色| 人人妻人人超人国产| 久久91视频在线| 美女激情吸奶舔下面一区二区二| 大黑鸡巴干嫩屄视频| 欧美图片 视频一区| 黄片国产A| 亚洲自拍3p| 国产福利视频论坛| 日韩精品影片| 欧美三级一区二区三级电影院| 大鸡巴xxx| znl66一区二区| 啪啪啪18日韩| 亚洲精产品99永久一区一区| 日本xx精品| 天天做天天爽天天爽| 麻豆精品三级视频| 亚洲入口一入口二久久久| 国产最近精品| 亚洲综合无码图片| 国产精品17页| 日本激情成人网站| 亚洲成人avv| 无码在线一区二区樱花视频| 一区二区月韩| 日韩A级伦理网站| 国产机巴日逼免费视频| 日本久久无码一区二区| 精品91色虎BB| 好吊妞这里有精品免费在线观看| 亚洲AV成人网站在线观看麻豆| 欧美日 韩不卡一区二区|